5TZN

Structure of the viral immunoevasin m12 (Smith) bound to the natural killer cell receptor NKR-P1B (B6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9436
Spacegroup name	C 1 2 1
Unit cell lengths	165.276, 61.912, 66.159
Unit cell angles	90.00, 101.92, 90.00

Refinement procedure

Resolution	44.800 - 2.600
R-factor	0.207
Rwork	0.204
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m9z 4pn6
RMSD bond length	0.008
RMSD bond angle	1.130
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.800	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.165	0.684
Number of reflections	19331
<I/σ(I)>		1.9
Completeness [%]		97.3
Redundancy		2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	41 % PEG400 0.1M Bis/Tris ph 6.3 0.4M sodium iodide