5TYX

DNA Polymerase Mu Product Complex, Mn2+ (15 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-24
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	59.908, 68.736, 109.962
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.128 - 1.948
R-factor	0.1869
Rwork	0.185
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.005
RMSD bond angle	0.729
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.950	5.290	1.950
Rmerge	0.101	0.048	0.658
Total number of observations	350600
Number of reflections	33656
<I/σ(I)>	9.9
Completeness [%]	99.1	97.2	91.9
Redundancy	10.4	10.7	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000