5TYU
DNA Polymerase Mu Reactant Complex, Mn2+ (4 min)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-17 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.155, 62.223, 118.588 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.366 - 2.048 |
| R-factor | 0.1881 |
| Rwork | 0.187 |
| R-free | 0.21250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m04 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.571 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 5.560 | 2.050 |
| Rmerge | 0.094 | 0.043 | 0.857 |
| Rmeas | 0.104 | ||
| Rpim | 0.044 | ||
| Total number of observations | 157686 | ||
| Number of reflections | 28596 | ||
| <I/σ(I)> | 7.8 | ||
| Completeness [%] | 99.0 | 99.6 | 98.3 |
| Redundancy | 5.5 | 5.4 | 5.5 |
| CC(1/2) | 0.998 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG4000 |
