5TYQ

Crystal structure of a holoenzyme methyltransferase involved in the biosynthesis of gentamicin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	66.692, 66.922, 68.849
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.903 - 2.163
R-factor	0.1969
Rwork	0.194
R-free	0.21600
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.179
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.903
High resolution limit [Å]	2.163
Rmerge	0.175
Number of reflections	16909
<I/σ(I)>	17.01
Completeness [%]	99.5
Redundancy	11.6
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	magnesium chloride, Tris, PEG 3350, PEG 400