5TX5

Rip1 Kinase ( flag 1-294, C34A, C127A, C233A, C240A) with GSK772

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.078
Spacegroup name	P 21 21 21
Unit cell lengths	42.774, 101.246, 129.753
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.660 - 2.560
R-factor	0.1995
Rwork	0.197
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.660
High resolution limit [Å]	2.560
Number of reflections	18589
<I/σ(I)>	13.4
Completeness [%]	98.0
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	23 % PEG 4000, 0.1 M ammonium acetate and 0.1 M HEPES pH 7.5.