5TWT
Characterization of class III peroxidase from switchgrass (Panicum virgatum)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2015-06-01 |
Detector | AGILENT EOS CCD |
Wavelength(s) | 0.99996 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.854, 59.432, 96.874 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.547 - 1.296 |
R-factor | 0.1349 |
Rwork | 0.135 |
R-free | 0.14530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sch |
RMSD bond length | 0.007 |
RMSD bond angle | 0.916 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.296 | |
Rmerge | 0.617 | |
Number of reflections | 75730 | |
<I/σ(I)> | 14.725 | 2.14 |
Completeness [%] | 97.6 | |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 2M (NH4)2SO4 |