5TWL
Structure of Maternal Embryonic Leucine Zipper Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.520, 68.300, 104.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.767 - 2.420 |
R-factor | 0.1983 |
Rwork | 0.196 |
R-free | 0.24910 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.770 |
High resolution limit [Å] | 2.420 |
Number of reflections | 15943 |
<I/σ(I)> | 12.52 |
Completeness [%] | 97.5 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | magnesium chloride, PEG 3350, BIS-TRIS |