5TVR

JMJD2A in complex with Ni(II) and alpha-Ketoglutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 2 21 21
Unit cell lengths	57.143, 101.122, 149.028
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.152 - 2.093
R-factor	0.1829
Rwork	0.181
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvh
RMSD bond length	0.005
RMSD bond angle	0.928
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.152	2.130
High resolution limit [Å]	2.090	2.090
Number of reflections	51526
<I/σ(I)>	23.7	2.9
Completeness [%]	99.8	99.8
Redundancy	7	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	17.5% PEG-3350 and 0.1 M citrate pH 6.0