5TVO
Crystal structure of Trypanosoma brucei AdoMetDC-delta26 monomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.669, 75.644, 87.915 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.006 - 1.481 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.20010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ep9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.987 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 51575 | |
| <I/σ(I)> | 29.8 | 1.4 |
| Completeness [%] | 98.2 | 79.2 |
| Redundancy | 9.4 | 4.8 |
| CC(1/2) | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25 % PEG 3350, 0.2 M Ammonium acetate, 0.1 M Bis-tris, pH 5.5 |
