5TV1

active arrestin-3 with inositol hexakisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	80
Detector technology	CCD
Collection date	2016-04-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 63
Unit cell lengths	97.575, 97.575, 76.938
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.030 - 2.400
R-factor	0.199
Rwork	0.197
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g4m
RMSD bond length	0.009
RMSD bond angle	1.285
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.030
High resolution limit [Å]	2.400
Number of reflections	15997
<I/σ(I)>	26.2
Completeness [%]	97.0
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	100 mM Succinate/Phosphate/Glycine pH 8.5 and 25% PEG 1500