5TRE
Zinc and the Iron Donor Frataxin Regulate Oligomerization of the Scaffold Protein to Form New Fe-S Cluster Assembly Centers
EM specimen
| Staining | Yes |
| Staining material | uranyl acetate |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 15.6 |
| Number of Particle | 4218 |
| Software | EMAN2,Situs,NAMD,Coot,DigitalMicrograph,UCSF Chimera |
| Symmetry | O |
| CTF correction | PHASE FLIPPING ONLY |
