5TR8
Crystal structure of vaccine-elicited pan- influenza H1N1 neutralizing murine antibody 441D6.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-05 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.463, 83.332, 83.160 |
| Unit cell angles | 90.00, 105.69, 90.00 |
Refinement procedure
| Resolution | 24.607 - 2.010 |
| R-factor | 0.1756 |
| Rwork | 0.174 |
| R-free | 0.19830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zpt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.856 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.607 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.553 |
| Number of reflections | 42733 | |
| <I/σ(I)> | 7.57 | 1.49 |
| Completeness [%] | 98.5 | 94.4 |
| Redundancy | 3.1 | 2.3 |
| CC(1/2) | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 273 | 12% PEG3350, 100 mM HEPES pH 7.5, 5 mM MgCl2, 5mM NiCl2 |
