5TQW
CryoEM reconstruction of human IKK1, open conformation 1
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.6 |
| Number of Particle | 230242 |
| Software | FindEM,FREALIGN,Rosetta,PHENIX,Leginon,CTFFIND,RELION |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
