5TPH
Crystal structure of a de novo designed protein homodimer with curved beta-sheet
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.210, 32.790, 86.480 |
| Unit cell angles | 90.00, 92.11, 90.00 |
Refinement procedure
| Resolution | 43.210 - 2.470 |
| R-factor | 0.2262 |
| Rwork | 0.223 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.502 |
| Data reduction software | iMOSFLM (7.1.0) |
| Data scaling software | Aimless (0.2.8) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (dev_1616) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.210 |
| High resolution limit [Å] | 2.470 |
| Number of reflections | 7955 |
| <I/σ(I)> | 22.1 |
| Completeness [%] | 99.9 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M potassium thiocyanate, pH 8 and 30% PEG MME 2000 |
