5TOS

Botrytis-induced kinase 1 (BIK1) from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	114.190, 72.090, 93.880
Unit cell angles	90.00, 108.41, 90.00

Refinement procedure

Resolution	33.543 - 2.350
R-factor	0.2395
Rwork	0.238
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oh4 3uim
RMSD bond length	0.002
RMSD bond angle	0.589
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.410
High resolution limit [Å]	2.350	2.350
Rmerge	0.081	0.575
Number of reflections	29382
<I/σ(I)>	9.73	1.93
Completeness [%]	96.6	95.1
Redundancy	2.92	2.9
CC(1/2)		0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7	293	245 mM ammonium tartrate, 12.5% PEG 3350 pH 7.0