5TLZ
Fructose-1,6-bisphosphate aldolase from rabbit muscle in complex with the inhibitor naphthalene 2,6-bisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-14 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.254, 103.638, 84.518 |
| Unit cell angles | 90.00, 98.67, 90.00 |
Refinement procedure
| Resolution | 48.345 - 1.970 |
| R-factor | 0.1294 |
| Rwork | 0.129 |
| R-free | 0.16610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qut |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.710 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.052 | 0.029 | 0.275 |
| Total number of observations | 283537 | ||
| Number of reflections | 87928 | ||
| <I/σ(I)> | 15 | ||
| Completeness [%] | 78.5 | 99.6 | 9.7 |
| Redundancy | 3.2 | 3.6 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 0.1M Sodium HEPES, 17.5% PEG 4000 |
