5TLI

THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-05-01
Detector	MAR scanner 300 mm plate
Spacegroup name	P 61 2 2
Unit cell lengths	94.230, 94.230, 131.030
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
Rwork	0.157
R-free	0.19300 *
Structure solution method	ISOMORPHOUS REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	2.100 *
Data reduction software	DENZO
Data scaling software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.240
High resolution limit [Å]	2.100	2.100
Rmerge	0.126	0.404
Number of reflections	20404
<I/σ(I)>	5.2	1.9
Completeness [%]	99.0	98
Redundancy	7.8	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5		4.6MM THERMOLYSIN, 45% DMSO, 50MM TRIS-HCL, 2.5M CSCL, PH 7.5, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	TLN	4.6 (mM)
2	1	1	DMSO	45 (%)
3	1	1	Tris-HCl	50 (mM)
4	1	1		2.5 (M)