5TLH
Fructose-1,6-bisphosphate aldolase from rabbit muscle in complex with the inhibitor 2-naphthol 6-bisphosphonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-14 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.569, 103.141, 84.865 |
| Unit cell angles | 90.00, 98.87, 90.00 |
Refinement procedure
| Resolution | 43.927 - 2.204 |
| R-factor | 0.1513 |
| Rwork | 0.150 |
| R-free | 0.18770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qut |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.858 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.250 |
| High resolution limit [Å] | 2.204 | 6.000 | 2.210 |
| Rmerge | 0.166 | 0.087 | |
| Number of reflections | 69137 | ||
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 96.8 | 99.8 | 94.8 |
| Redundancy | 4.8 | 5.6 | 4.4 |
| CC(1/2) | 0.988 | 0.682 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 0.1M Sodium HEPES, 17.5% PEG 4000 |
