5TLA

Crystal structure of mouse ISG15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 1 2 1
Unit cell lengths	85.759, 60.161, 172.658
Unit cell angles	90.00, 93.12, 90.00

Refinement procedure

Resolution	42.183 - 3.244
R-factor	0.2589
Rwork	0.256
R-free	0.30910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jze
RMSD bond length	0.002
RMSD bond angle	0.567
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_2420)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.244	8.810	3.250
Rmerge	0.052	0.012	0.375
Number of reflections	27964
<I/σ(I)>	12
Completeness [%]	98.4	98.4	88
Redundancy	2.4	2.3	2.1
CC(1/2)		0.999	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	293	0.2 M ammonium sulfate, 0.1 M sodium acetate, 12% PEG4000 and 0.2 M sodium malonate