5TLA
Crystal structure of mouse ISG15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 85.759, 60.161, 172.658 |
| Unit cell angles | 90.00, 93.12, 90.00 |
Refinement procedure
| Resolution | 42.183 - 3.244 |
| R-factor | 0.2589 |
| Rwork | 0.256 |
| R-free | 0.30910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jze |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.567 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2420) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
| High resolution limit [Å] | 3.244 | 8.810 | 3.250 |
| Rmerge | 0.052 | 0.012 | 0.375 |
| Number of reflections | 27964 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 98.4 | 98.4 | 88 |
| Redundancy | 2.4 | 2.3 | 2.1 |
| CC(1/2) | 0.999 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 293 | 0.2 M ammonium sulfate, 0.1 M sodium acetate, 12% PEG4000 and 0.2 M sodium malonate |
