5TJ4
Gasdermin-B C-terminal domain containing the polymorphism residues Gly299:Pro306 fused to maltose binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-11-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.599, 274.569, 174.019 |
| Unit cell angles | 90.00, 96.09, 90.00 |
Refinement procedure
| Resolution | 173.040 - 3.500 |
| R-factor | 0.2148 |
| Rwork | 0.213 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tib |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.678 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 173.040 |
| High resolution limit [Å] | 3.500 |
| Rmerge | 0.094 |
| Number of reflections | 99563 |
| <I/σ(I)> | 8.5 |
| Completeness [%] | 99.7 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 295 | 2.1 M sodium malonate |
