5TIB
Gasdermin-B C-terminal domain containing the polymorphism residues Arg299:Ser306 fused to maltose binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-04-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 104.060, 104.060, 252.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 96.190 - 2.600 |
R-factor | 0.1861 |
Rwork | 0.184 |
R-free | 0.23310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 3H4Z |
RMSD bond length | 0.016 |
RMSD bond angle | 1.490 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 96.200 |
High resolution limit [Å] | 2.600 |
Rmerge | 0.112 |
Number of reflections | 41354 |
<I/σ(I)> | 10.6 |
Completeness [%] | 99.9 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 298 | 2.2 M sodium malonate, and 9 mM hexamine CoCl3 |