5THC
Crystal structure of H10 hemagglutinin mutant (T193D-Q226L-G228S) from Jiangxi-Donghu (2013) H10N8 influenza virus in complex with 6'-SLNLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.186, 244.451, 70.758 |
| Unit cell angles | 90.00, 111.67, 90.00 |
Refinement procedure
| Resolution | 37.778 - 2.792 |
| R-factor | 0.1937 |
| Rwork | 0.191 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tgo |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.562 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 48559 | |
| <I/σ(I)> | 9.2 | 2.1 |
| Completeness [%] | 97.6 | 98.8 |
| Redundancy | 3.3 | 3.1 |
| CC(1/2) | 0.552 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 0.2 M NaCl, 10% (w/v) PEG 8000, 0.1M CHES pH 9.5 |
