5TH5
Crystal Structure of QueE from Bacillus subtilis with 6-carboxypterin-5'-deoxyadenosyl ester bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.879, 136.835, 141.187 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.504 - 2.407 |
R-factor | 0.2187 |
Rwork | 0.217 |
R-free | 0.24280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tgs |
RMSD bond length | 0.008 |
RMSD bond angle | 2.124 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.504 | 2.460 |
High resolution limit [Å] | 2.407 | 2.407 |
Number of reflections | 40846 | |
<I/σ(I)> | 10.7 | 2.1 |
Completeness [%] | 98.8 | 93.8 |
Redundancy | 3.2 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 294 | 1 M trisodium citrate (pH 4.2), 1.5 M Ammonium Sulfate |