5TF1

Structure of chimeric 02-CC Fab, a VRC01-like germline antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	44.579, 58.676, 70.408
Unit cell angles	90.00, 103.85, 90.00

Refinement procedure

Resolution	34.831 - 1.858
R-factor	0.2112
Rwork	0.210
R-free	0.23250
RMSD bond length	0.002
RMSD bond angle	0.590
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	34.831
High resolution limit [Å]	1.858
Number of reflections	27409
<I/σ(I)>	16.4
Completeness [%]	91.8
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG 3350, 0.1 M Hepes pH 7.5