5TF1
Structure of chimeric 02-CC Fab, a VRC01-like germline antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.579, 58.676, 70.408 |
Unit cell angles | 90.00, 103.85, 90.00 |
Refinement procedure
Resolution | 34.831 - 1.858 |
R-factor | 0.2112 |
Rwork | 0.210 |
R-free | 0.23250 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.590 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.831 |
High resolution limit [Å] | 1.858 |
Number of reflections | 27409 |
<I/σ(I)> | 16.4 |
Completeness [%] | 91.8 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 3350, 0.1 M Hepes pH 7.5 |