5TDZ

TEV Cleaved Human ATP Citrate Lyase Bound to Tartrate and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	55.672, 84.291, 195.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.380 - 2.000
R-factor	0.177
Rwork	0.175
R-free	0.22200
RMSD bond length	0.013
RMSD bond angle	1.123
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.380
High resolution limit [Å]	2.000
Number of reflections	61452
<I/σ(I)>	12
Completeness [%]	97.6
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	294	13% P3350, 100 mM TrisHCl pH 8.8, 100 mM ammonium chloride