5TDA
Crystal structure of the UBR-box domain from UBR2 in complex with RLWS N-degron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.6362 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.702, 37.158, 57.474 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.028 - 0.790 |
| R-factor | 0.1205 |
| Rwork | 0.120 |
| R-free | 0.12810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ny3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.059 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.028 | 0.810 |
| High resolution limit [Å] | 0.790 | 0.790 |
| Number of reflections | 65534 | |
| <I/σ(I)> | 50.69 | |
| Completeness [%] | 96.3 | 70 |
| Redundancy | 5.4 | 2.6 |
| CC(1/2) | 0.674 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M MES pH 6.5 and 20% PEG 10000 |
