5TD6

C. elegans FOG-3 BTG/Tob domain - H47N, C117A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-15
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.953720
Spacegroup name	P 31 2 1
Unit cell lengths	64.992, 64.992, 133.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.220 - 2.034
R-factor	0.1816
Rwork	0.177
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d5r
RMSD bond length	0.009
RMSD bond angle	1.103
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.220	2.106
High resolution limit [Å]	2.034	2.034
Rmerge	0.050	0.738
Number of reflections	21617
<I/σ(I)>	24.12	2.56
Completeness [%]	99.8	98.18
Redundancy	6.8	7
CC(1/2)	1.000	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	Mg Acetate, Na Citrate, PEG 10000