5TCK
Second Bromodomain from Leishmania donovani LdBPK.091320 complexed with Bromosporine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-22 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.245, 74.086, 106.412 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.840 - 1.950 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.620 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | Arcimboldo |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.950 |
Number of reflections | 20521 |
<I/σ(I)> | 24.5 |
Completeness [%] | 99.7 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | The protein was crystallized at 293 K with 1% w/v Tryptone, 0.001 M Sodium azide, 0.05 M HEPES sodium pH 7.0, 12% w/v Polyethylene glycol 3,350 with Bromosporine using the sitting drop method. |