5TBY
HUMAN BETA CARDIAC HEAVY MEROMYOSIN INTERACTING-HEADS MOTIF OBTAINED BY HOMOLOGY MODELING (USING SWISS-MODEL) OF HUMAN SEQUENCE FROM APHONOPELMA HOMOLOGY MODEL (PDB-3JBH), RIGIDLY FITTED TO HUMAN BETA-CARDIAC NEGATIVELY STAINED THICK FILAMENT 3D-RECONSTRUCTION (EMD-2240)
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI/PHILIPS CM120T |
| Electron Gun | LAB6 |
| Accelerating Voltage (kV) | 120 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 20 |
| Number of Particle | 15504 |
| Software | EMAN,ITERATIVE HELICAL REAL SPACE RECONSTRUCTION (EGELMAN, 2000),UCSF Chimera |
| Symmetry | C4 |
| CTF correction | NONE |
