5TBW

Crystal structure of chlorolissoclimide bound to the yeast 80S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.148
Spacegroup name	P 1 21 1
Unit cell lengths	304.800, 288.200, 437.680
Unit cell angles	90.00, 98.71, 90.00

Refinement procedure

Resolution	99.856 - 3.000
R-factor	0.1944
Rwork	0.194
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v88
RMSD bond length	0.006
RMSD bond angle	1.043
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.100
High resolution limit [Å]	3.000	75.000	3.000
Rmerge	0.204	0.021	1.124
Rmeas	0.216	0.023	1.243
Total number of observations	13350896
Number of reflections	1484770	51	138249
<I/σ(I)>	10.63	37.88	1.39
Completeness [%]	100.0	83.6	99.6
Redundancy	9
CC(1/2)	0.997	1.000	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	Tris-acetate pH7.0, potassium thiocyanate, magnesium acetate, glycerol, spermidine, PEG 20000