5TB9
Precatalytic ternary complex of Human DNA Polymerase Beta in closed conformation With Gapped DNA substrate incoming (-)FTC-TP and Mn2+.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2014-11-07 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.190, 82.310, 54.280 |
| Unit cell angles | 90.00, 110.97, 90.00 |
Refinement procedure
| Resolution | 46.870 - 2.490 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rpx |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.151 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.870 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.107 | 0.545 |
| Number of reflections | 14580 | |
| <I/σ(I)> | 8.3 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 50 MM IMIDAZOLE, 350 MM SODIUM ACETATE, 17% PEG3350, PH 8.0 |
