5T8R

Crystal structure of human BAZ2A PHD zinc finger in complex with unmodified H3 10-mer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 43 21 2
Unit cell lengths	72.607, 72.607, 99.431
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.620 - 2.400
R-factor	0.1877
Rwork	0.185
R-free	0.23530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qf2
RMSD bond length	0.011
RMSD bond angle	1.510
Data reduction software	XDS
Data scaling software	Aimless (0.5.15)
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.620	45.620	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.113	0.057	0.782
Number of reflections	10901
<I/σ(I)>	9.3
Completeness [%]	99.6	95.7	100
Redundancy	5.4	4.5	5.8
CC(1/2)	0.994	0.999	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	2.2 M Na/K phosphate