5T83
Structure of a guanidine-I riboswitch from S. acidophilus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.999946, 1.10532, 1.10482 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 49.091, 49.091, 246.341 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.710 |
R-factor | 0.202 |
Rwork | 0.200 |
R-free | 0.22600 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.074 | 0.712 |
Number of reflections | 15638 | |
<I/σ(I)> | 15.2 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.9 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 4.6 | 296 | RNA solution: 130 uM RNA in 10 mM MgCl2, 10 mM KCl, and 10 mM HEPES-KOH, pH 7.5; Reagent: 32% MPD, 40 mM SrCl2, 12 mM spermine, 40 mM sodium acetate, pH 4.6, and 0.8 mM iridium (III) hexamine; RNA solution and reagent mixed 1:1 |
1 | MICROBATCH | 4.6 | 296 | RNA solution: 130 uM RNA in 10 mM MgCl2, 10 mM KCl, and 10 mM HEPES-KOH, pH 7.5; Reagent: 32% MPD, 40 mM SrCl2, 12 mM spermine, 40 mM sodium acetate, pH 4.6, and 0.8 mM iridium (III) hexamine; RNA solution and reagent mixed 1:1 |