5T7G
Crystal Structure of Murine MHC-I H-2Dd in complex with Murine Beta2-Microglobulin and a Variant of Peptide (PT9) of HIV gp120 MN Isolate (IGPGRAFYT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0333 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.243, 120.496, 143.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.966 - 1.961 |
R-factor | 0.1969 |
Rwork | 0.195 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kd7 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.194 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.060 | 0.972 |
Number of reflections | 63236 | |
<I/σ(I)> | 12.9 | 1.3 |
Completeness [%] | 99.5 | 98.6 |
Redundancy | 4 | 3.9 |
CC(1/2) | 0.999 | 0.546 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 12% PEG 10000, 0.1M MES buffer |