5T77

Crystal structure of the MOP flippase MurJ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	94.163, 99.573, 74.660
Unit cell angles	90.00, 112.91, 90.00

Refinement procedure

Resolution	34.192 - 2.000
R-factor	0.19
Rwork	0.189
R-free	0.21410
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.546
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.000
Number of reflections	41992
<I/σ(I)>	9.47
Completeness [%]	98.0
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		295	PEG400, calcium chloride