5T6L
Crystal structure of 10E8 Fab in complex with the MPER epitope scaffold T117v2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-06-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03314 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.100, 158.970, 99.690 |
| Unit cell angles | 90.00, 97.98, 90.00 |
Refinement procedure
| Resolution | 47.140 - 2.100 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g6f 3lf6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.877 |
| Data reduction software | XDS (VERSION March 30, 2013 BUILT=20130527) |
| Data scaling software | XSCALE (VERSION July 4, 2012 BUILT=20130527) |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX ((1.10_2155: 000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.142 | 2.150 |
| High resolution limit [Å] | 2.096 | 2.100 |
| Rmerge | 0.630 | |
| Number of reflections | 72210 | |
| <I/σ(I)> | 9.3 | 1.6 |
| Completeness [%] | 97.5 | 91.9 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.1 M Hepes pH 7.0, 15% PEG 20,000 |
