5T66
Crystal Structure of CTX-M-15 with 1C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-15 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 43.463, 76.106, 149.375 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.678 - 1.951 |
| R-factor | 0.2022 |
| Rwork | 0.200 |
| R-free | 0.23410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hbt |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.673 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.680 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.124 | |
| Number of reflections | 36639 | |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 99.0 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M HEPES, pH 7.5, 70% v/v 2,4-methyl pentanediol |
