5T4N
Crystal Structure of Human Protocadherin-15 EC3-5 D414A Variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 65 |
Unit cell lengths | 105.655, 105.655, 193.767 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.380 - 2.701 |
Rwork | 0.202 |
R-free | 0.23870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5t4m |
RMSD bond length | 0.012 |
RMSD bond angle | 0.754 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1819) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.380 | 2.798 |
High resolution limit [Å] | 2.701 | 2.701 |
Rmerge | 0.184 | 3.498 |
Number of reflections | 33423 | |
<I/σ(I)> | 9.18 | 0.6 |
Completeness [%] | 99.0 | 99 |
Redundancy | 7 | 7 |
CC(1/2) | 0.996 | 0.171 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 0.1 M Sodium Cacodylate pH 5.8, 150 mM Calcium Chloride |