5T4K

PLP and GABA Trigger GabR-Mediated Transcription Regulation in Bacillus subsidies via External Aldimine Formation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	96.630, 127.530, 67.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.797 - 2.245
R-factor	0.1882
Rwork	0.186
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t4j
RMSD bond length	0.003
RMSD bond angle	0.604
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (dev_1563)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.800
High resolution limit [Å]	2.245
Number of reflections	20140
<I/σ(I)>	12.6
Completeness [%]	96.2
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris (pH: 8.5) and 20% ethanol