5T48

Crystal structure of the D. melanogaster eIF4E-eIF4G complex without lateral contact

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99992
Spacegroup name	P 21 21 21
Unit cell lengths	45.622, 66.196, 75.618
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.063 - 2.190
R-factor	0.2096
Rwork	0.208
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ue8
RMSD bond length	0.003
RMSD bond angle	0.478
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2152))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.620	2.260
High resolution limit [Å]	2.190	2.190
Number of reflections	12336
<I/σ(I)>	17	2.3
Completeness [%]	100.0	99.6
Redundancy	12.6	11.4
CC(1/2)	0.999	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.1 M TrisHCl pH 8.0, 0.01 M ZnCl2, 20% w/v PEG6000