5T3Z

3.5 Angstrom Crystal Structure of a Fully and Natively Glycosylated BG505 SOSIP.664 HIV-1 Env Trimer in Complex with the Broadly Neutralizing Antibodies IOMA and 10-1074

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	H 3
Unit cell lengths	217.258, 217.258, 154.935
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.631 - 3.500
R-factor	0.2781
Rwork	0.275
R-free	0.29840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t3x
RMSD bond length	0.004
RMSD bond angle	1.100
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.631	3.670
High resolution limit [Å]	3.500	3.500
Rmerge	0.680	9.290
Rpim	0.090	1.210
Number of reflections	34373
<I/σ(I)>	12.6	1.6
Completeness [%]	100.0	100
Redundancy	61.1	59.7
CC(1/2)	0.990	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM ammonium citrate tribasic pH 7.0 100 mM imidazole pH 7.0 20% PEG MME 2,000