5T3T

Ebola virus VP30 CTD bound to nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	85.875, 116.784, 95.038
Unit cell angles	90.00, 108.52, 90.00

Refinement procedure

Resolution	90.120 - 2.200
R-factor	0.21593
Rwork	0.214
R-free	0.24925
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i8b
RMSD bond length	0.013
RMSD bond angle	1.578
Data reduction software	XDS
Data scaling software	XDS (January 10, 2014)
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.120	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.120	0.950
Number of reflections	89012
<I/σ(I)>	7.3	1.4
Completeness [%]	98.6	93
Redundancy	3	3
CC(1/2)	0.990	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.9	298	0.3 uL of 20 mg/mL protein mixed with 0.3 uL of 2.2 M ammonium sulfate, 100 mM sodium acetate pH 4.9