5T1H
Crystal structure of CK2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 127.090, 127.090, 124.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 89.860 - 2.110 |
R-factor | 0.179 |
Rwork | 0.178 |
R-free | 0.20300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h8b |
RMSD bond length | 0.010 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 127.000 | 2.360 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmerge | 0.187 | |
Number of reflections | 59135 | |
<I/σ(I)> | 18.1 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.7 | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 22-26% PEG 6K, 200 mM ammonium sulfate, 100 mM MES |