5SZZ
Novel Structural Insights into GDP-Mediated Regulation of Acyl-CoA Thioesterases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 152.620, 152.620, 152.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.120 - 2.300 |
| R-factor | 0.19085 |
| Rwork | 0.190 |
| R-free | 0.21238 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ien |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.120 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.013 | 0.050 |
| Number of reflections | 52676 | |
| <I/σ(I)> | 40.43 | 14.06 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 22 | 21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 100 mM Tris pH 8.5 and 2 M ammonium phosphate |
