5SZM

Protocadherin gamma A8 extracellular cadherin domains 1-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	I 41 2 2
Unit cell lengths	257.560, 257.560, 105.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.938 - 3.600
R-factor	0.2122
Rwork	0.211
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zps
RMSD bond length	0.014
RMSD bond angle	0.564
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PH
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.510	3.970
High resolution limit [Å]	3.600	3.600
Rmerge	0.172	0.913
Number of reflections	20729
<I/σ(I)>	8.6	2.3
Completeness [%]	99.8	100
Redundancy	7.1	7.3
CC(1/2)	0.998	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.5	295	11% isopropanol, 50 mM sodium chloride, 0.1 M Hepes pH 7.5