5SZL
Protocadherin gamma A1 extracellular cadherin domains 1-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 107.867, 107.867, 463.081 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.960 - 4.200 |
| R-factor | 0.2879 |
| Rwork | 0.287 |
| R-free | 0.31360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zi9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.822 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.540 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.379 | 2.646 |
| Number of reflections | 23885 | |
| <I/σ(I)> | 3.1 | 1.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.991 | 0.318 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7 | 295 | 8% (w/v) PEG8000, 16% ethylene glycol, 20% Morpheus Amino Acids (Molecular Dimensions), 0.1 M Morpheus Buffer System 2 (Hepes/MOPS buffer; Molecular Dimensions) pH 7.0 |
