5SZK
Structure of human N-terminally engineered Rab1b in complex with the bMERB domain of Mical-cL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91908 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.230, 117.030, 139.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.800 - 2.800 |
R-factor | 0.2102 |
Rwork | 0.207 |
R-free | 0.26070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nkv 5szg |
RMSD bond length | 0.010 |
RMSD bond angle | 1.178 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10-2155_1692: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.800 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.076 | 1.110 |
Number of reflections | 12904 | |
<I/σ(I)> | 22.8 | 2.45 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.2 | 13.8 |
CC(1/2) | 0.757 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M bis-tris pH 7.5, 0.2M sodium malonate and 20% (w/v) PEG3350 |