5SZI

Structure of human Rab8a in complex with the bMERB domain of Mical-cL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00009
Spacegroup name	C 2 2 21
Unit cell lengths	62.400, 122.400, 139.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.952 - 2.850
R-factor	0.2396
Rwork	0.237
R-free	0.28840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lhw 5szg
RMSD bond length	0.009
RMSD bond angle	1.175
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10-2155_1692: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.089	0.780
Number of reflections	12810
<I/σ(I)>	16.45	3.2
Completeness [%]	99.9	100
Redundancy	12.7	13.5
CC(1/2)		0.507

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M bis tris propane 0.2M tri sodium citrate 20% PEG 3350