5SZH

Structure of human Rab1b in complex with the bMERB domain of Mical-cL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	I 2 2 2
Unit cell lengths	61.750, 129.380, 129.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.826 - 2.300
R-factor	0.1807
Rwork	0.179
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nkv 5szg
RMSD bond length	0.008
RMSD bond angle	1.104
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.830	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.105	0.740
Number of reflections	23533
<I/σ(I)>	16.27	3.89
Completeness [%]	100.0	100
Redundancy	13.1	13.4
CC(1/2)		0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20-22% PEG3350 0.1M bis-Tris Propane 0.2M tri-Sodium Citrate