5SZD

Crystal structure of Aquifex aeolicus Hfq at 1.5A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97920
Spacegroup name	P 1
Unit cell lengths	63.460, 66.060, 66.100
Unit cell angles	60.05, 83.94, 77.17

Refinement procedure

Resolution	46.351 - 1.494
R-factor	0.1351
Rwork	0.132
R-free	0.16960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u1s
RMSD bond length	0.005
RMSD bond angle	0.745
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10-2155_1309: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	57.270
High resolution limit [Å]	1.490
Number of reflections	138120
<I/σ(I)>	14
Completeness [%]	93.7
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	sodium cacodylate, PEG 8000, MPD, 0.1 M hexamminecobalt(III) chloride